Journal: International Journal of Molecular Sciences
Article Title: Suppressive Potential of Ethanolic Extracts of Parkia speciosa Hassk. Empty Pods Against Colon Cancer Cell Migration and Invasion
doi: 10.3390/ijms27042072
Figure Lengend Snippet: Western blot showing the effects of the PSET on the abundance of proteins associated with the downstream EMT pathway. Representative Western blot image showing the amount of MMP2, MMP9 and GAPDH proteins in control (untreated cells) and treatment (PSET) groups. The molecular weight (MW) of each protein is provided. Quantitative analysis of protein levels following Western blot analysis. Data in histograms are presented as the means ± SDs from three independent experiments ( n = 3), with asterisks indicating statistical differences: * p < 0.05.
Article Snippet: The primary antibodies used were rabbit polyclonal anti-human MMP2 (#4022), MMP9 (#3852), N-cadherin (#4061), and GAPDH (#2118) (dilution 1:500; Cell Signaling Technology, Danvers, MA, USA).
Techniques: Western Blot, Control, Molecular Weight